Scanning Electron Microscope (Hitachi S3200N)

Accelerating Voltage
  • 0.3 to 30kV
Filament
  • Tungsten
Vacuum
  • VP mode 1.3-270Pa
Detectors
  • Imaging - Everhart Thornley & Robinson BSE Detectors
  • Oxford Instruments EDS7021 Oxford INCA Energy E2H X-ray Energy Dispersive Spectrometer (EDS) system with Silicon Drifted detector
Magnification
  • 20-300,000x
SEM Resolution
  • High Vacuum Mode 3.5nm
  • Low Vacuum Mode 5.5nm

The scanning electron microscope (SEM) has many advantages over traditional microscopes. The SEM has a large depth of field, which allows more of a specimen to be in focus at one time.

The SEM also has much higher resolution, so closely spaced specimens can be magnified at much higher levels. The magnification range of SEM is ~20 to 250,000x, with a practical operational magnification of ~60,000x.

Because the SEM uses electromagnets rather than lenses, the researcher has much more control in the degree of magnification. All of these advantages, as well as the actual strikingly clear images, make the scanning electron microscope one of the most useful instruments in research today.

The HITACHI S3200N has a large specimen chamber, a back scattered electron detector and a variable pressure mode (VP-SEM). This means that the equipment can be operated in either a high vacuum mode (~0.001 Pa) or a low vacuum mode (1 to 270 Pa) allowing the examination of non-conducting samples with no special sample preparation.

The ultra-thin window Energy Dispersive X-ray (EDX) detector makes qualitative/quantitative elemental analysis also possible.

 
SEM and EDS analysis of a metal shard found in an eye.

 

Explorer Atomic Force Microscopes (AFM)

The Scanning Probe Microscope (SPM) gives three-dimensional analysis of surface properties in the micro and nano metric range. Depending on the princple, the SPM can map topography (AFM and STM), thermal (sThM), electrical (EFM) and magnetic properties (MFM).

Scanners

 Scanner X,Y Range Z Range
 AFM (dry) 2μm 0.8μm
 AFM (dry) 100μm 8μm
 AFM (liquid) 2μm 0.8μm
 MFM/EFM 100μm 8μm
 STM Long range  

 

 
AFM Nickel gratingAFM/MFM particulate PTCO media

 

 

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